Physiological and biochemical characterization of the soluble formate dehydrogenase, a molybdoenzyme from Alcaligenes eutrophus
نویسندگان
چکیده
منابع مشابه
Biochemical and molecular characterization of the Alcaligenes eutrophus pyruvate dehydrogenase complex and identification of a new type of dihydrolipoamide dehydrogenase.
Sequence analysis of a 6.3-kbp genomic EcoRI-fragment of Alcaligenes eutrophus, which was recently identified by using a dihydrolipoamide dehydrogenase-specific DNA probe (A. Pries, S. Hein, and A. Steinbüchel, FEMS Microbiol. Lett. 97:227-234, 1992), and of an adjacent 1.0-kbp EcoRI fragment revealed the structural genes of the A. eutrophus pyruvate dehydrogenase complex, pdhA (2,685 bp), pdhB...
متن کامل+)-Lactate Dehydrogenase from the Strict Aerobe Alcaligenes eutrophus
The L( +)-lactate dehydrogenase (EC 1.1.1.27) of Alcaligenes eutrophus catalyzes the NADH-dependent reduction of pyruvate and a few other 2-oxoacids. The K,,, values for NADH, NAD, pyruvate and L( +)-lactate are 0.075 mM, 0.130 mM, 0.820 mM and 7.10 mM, respectively. The reaction follows a rapid equilibrium ordered bi-bi mechanism and involves the formation of a dead-end EBQ complex. The compet...
متن کاملCloning of the Alcaligenes eutrophus alcohol dehydrogenase gene.
Mutants of Alcaligenes eutrophus which are altered with respect to the utilization of 2,3-butanediol and acetoin were isolated after transposon mutagenesis. The suicide vehicle pSUP5011 was used to introduce the drug resistance transposable element Tn5 into A. eutrophus. Kanamycin-resistant transconjugants of the 2,3-butanediol-utilizing parent strains CF10141 and AS141 were screened for mutant...
متن کاملPrimary sequence and evidence for a physiological function of the flavohemoprotein of Alcaligenes eutrophus.
The flavohemoprotein (FHP) encoding gene of the strictly respiratory Gram-negative bacterium Alcaligenes eutrophus was isolated from a megaplasmid library by using FHP-specific antibodies and oligonucleotide probes based on the amino-terminal polypeptide sequence of FHP, determined previously (Zhu, H., and Riggs, A. F. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5015-5019). The fhp gene codes for ...
متن کاملPurification and characterization of aromatic amine dehydrogenase from Alcaligenes xylosoxidans.
Aromatic amine dehydrogenase was purified and characterized from Alcaligenes xylosoxidans IFO13495 grown on beta-phenylethylamine. The molecular mass of the enzyme was 95.5 kDa. The enzyme consisted of heterotetrameric subunits (alpha2beta2) with two different molecular masses of 42.3 kDa and 15.2 kDa. The N-terminal amino acid sequences of the alpha-subunit (42.3-kDa subunit) and the beta-subu...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1993
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.175.15.4719-4728.1993